Data for the Constructed Mutant: YACFd

General Information

Name of the MutantYACFd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size999 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YACFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) yacF-F: cgaggcagatctAAACCGGGTTGTGCTCGC
Reverse Primer:(cgaggcagatctctcgag tag) yacF-R: cgaggcagatctctcgagCGAGAAGCCATTTGGCTCC
Length of the Cloned Region: 30 to 355 Length: 326
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YACFd