Data for the Constructed Mutant: YACFd
General Information
Name of the Mutant
YACFd (disruption)
Host
168
Used Vector
pMutinT3
Used Enzyme Sites
BglII & XhoI, BglII
Gene Size
999 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YACFd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
cgaggcagatct
tag)
yacF-F:
cgaggcagatctAAACCGGGTTGTGCTCGC
Reverse Primer:
(
cgaggcagatctctcgag
tag)
yacF-R:
cgaggcagatctctcgagCGAGAAGCCATTTGGCTCC
Length of the Cloned Region: 30 to 355 Length: 326
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:yes
Enzyme:
Probe used:
Figure
Comment: ;.
1st Level Phenotype Analysis of
YACFd