Data for the Constructed Mutant: CYSKd

General Information

Name of the MutantCYSKd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size924 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for CYSKd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) cysK-F: cgaggcagatctAATTAATTGGGAATACGC
Reverse Primer:(cgaggcagatctctcgag tag) cysK-R: cgaggcagatctctcgagGCTCAGCACCATATGCGC
Length of the Cloned Region: 29 to 340 Length: 312
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of CYSKd