Data for the Constructed Mutant: YACDd

General Information

Name of the MutantYACDd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size891 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YACDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) yacDF: cgaggcagatctTGGACCATTATTTTAGGG
Reverse Primer:(cgaggcagatctctcgag tag) yacDR: cgaggcagatctctcgagCTTTCCACTCTTTCTCCG
Length of the Cloned Region: 19 to 349 Length: 331
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YACDd