Data for the Constructed Mutant: YACBd

General Information

Name of the MutantYACBd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size699 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YACBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagc tag) yacB-F: aagaagcTTATCGATGTGGGGAACACC
Reverse Primer:(ggaggat tag) yacB-R: ggaggatCCATCTATCTGTTCAAACAT
Length of the Cloned Region: 11 to 176 Length: 166
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YACBd