Data for the Constructed Mutant: FTSHd

General Information

Name of the MutantFTSHd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size1911 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for FTSHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(agctaaagatc tag) ftsH-F: agctaaagatcTCGGGTCTTCCGTAATACC
Reverse Primer:(tgtggcagatctc tag) ftsH-R: tgtggcagatctcGAGTCAAAAAGTATTGATCTTTG
Length of the Cloned Region: 6 to 235 Length: 230
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of FTSHd