Data for the Constructed Mutant: YACAd

General Information

Name of the MutantYACAd (disruption)
Host168
Used VectorpMutin1
Used Enzyme SitesBglII & HindIII, BglII
Gene Size1416 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YACAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) yacA-F: cgaggcagatctTACCTGCTCATTGTGAGG
Reverse Primer:(cgaggcagatctaagctt tag) yacA-R: cgaggcagatctaagcttACGGCCTGATGATCCGCC
Length of the Cloned Region: -26 to 493 Length: 520
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YACAd