Data for the Constructed Mutant: YABSd

General Information

Name of the MutantYABSd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size735 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YABSd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) yabS-F: cgaggcagatctCAAATTTTGCTGATAACG
Reverse Primer:(cgaggcagatctctcgag tag) yabS-R: cgaggcagatctctcgagTTCCGGAGAAAGCTCTTC
Length of the Cloned Region: 22 to 345 Length: 324
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YABSd