Data for the Constructed Mutant: SPOIIEd

General Information

Name of the MutantSPOIIEd (disruption)
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size2481 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for SPOIIEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) spoIIE-F: cgaggcagatctGTGAACGGGCCAATGGCG
Reverse Primer:(cgaggcagatctctcgag tag) spoIIE-R: cgaggcagatctctcgagTTTGGGCATAAACAAACCC
Length of the Cloned Region: 22 to 442 Length: 421
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of SPOIIEd