Data for the Constructed Mutant: YABRd

General Information

Name of the MutantYABRd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size384 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YABRd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagct tag) yabR-F: aagaagctTCGATTGAAGTTGGCAGCAA
Reverse Primer:(ggaggatc tag) yabR-R: ggaggatcCACTAATGTGAACGAGACCG
Length of the Cloned Region: 4 to 109 Length: 106
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YABRd