Data for the Constructed Mutant: YABQd

General Information

Name of the MutantYABQd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size633 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YABQd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yabQ-F: aagaagcttACGACACAATTCTATACAAT
Reverse Primer:(ggagg tag) yabQ-R: ggaggATCCAGAAAAGAATATCATG
Length of the Cloned Region: 10 to 149 Length: 140
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YABQd