Data for the Constructed Mutant: YABOdd

General Information

Name of the MutantYABOdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-XhoI & XhoI-BamHI
Gene Size258 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YABOdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcg tag) yabO-UF:ctcctcgAGTGAAAAAATGGTCCGAAG
Reverse Primer:(ggaggatc tag) yabO-UR:ggaggatcCTCATATCTATGATCTCCTC
Downstream:
Forward Primer:(aagaagct tag) yabO-DF:aagaagctTCGGCGAATAGGCTTGTTCT
Reverse Primer:(ctcctcg tag) yabO-DR: ctcctcgAGCCATCTGGCTGTTTTGGC
Linearization:
Length of the Deletion: 5 to 251 Length: 247


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YABOdd