Data for the Constructed Mutant: YABNd

General Information

Name of the MutantYABNd (disruption)
Host168
Used VectorpMutinT3
Used Enzyme SitesBglII & XhoI, BglII
Gene Size1467 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YABNd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) yabN-F: cgaggcagatctTCGTCGGACTTGGTGCCG
Reverse Primer:(cgaggcagatctctcgag tag) yabN-R: cgaggcagatctctcgagCCGCGGACAATGTGCCGG
Length of the Cloned Region: 20 to 445 Length: 426
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YABNd