Data for the Constructed Mutant: YABKdd

General Information

Name of the MutantYABKdd (deletion (double-cross))
Host168
Used VectorpMutin2
Used Enzyme SitesEcoRI-XhoI & XhoI-BamHI
Gene Size228 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YABKdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctc tag) yabK-UF:ctcctcGAGCTGAATCAAAATAAATT
Reverse Primer:(ggaggatc tag) yabK-UR:ggaggatcCAATAATAATGCAAAGCCAT
Downstream:
Forward Primer:(gaagaa tag) yabK-DF:gaagaaTTCAATAAAAAGCTTTGGTG
Reverse Primer:(ctcctc tag) yabK-DR: ctcctcGAGCATCTGGCTGCTTTTCC
Linearization:
Length of the Deletion: 20 to 224 Length: 205


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YABKdd