Data for the Constructed Mutant: CTCd

General Information

Name of the MutantCTCd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size612 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for CTCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaag tag) ctc-F: aagaagCTTTAACGGCAAAAGAAAGA
Reverse Primer:(ggaggat tag) ctc-R: ggaggatCCTCAAGCGTAATGACTGCA
Length of the Cloned Region: 8 to 190 Length: 183
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of CTCd