Data for the Constructed Mutant: PRSp

General Information

Name of the MutantPRSp (promoter fusion)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size951 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for PRSp (promoter fusion)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) prs(SD)-F: aagaagcttGACGATTATGTGAAAAATATTC
Reverse Primer:(ggagg tag) prs-R: ggaggATCCGCGAATACTTTCTTCG
Length of the Cloned Region: -55 to 169 Length: 225
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of PRSp