Data for the Constructed Mutant: YABHp

General Information

Name of the MutantYABHp (promoter fusion)
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII & XhoI, BglII
Gene Size867 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YABHp (promoter fusion)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagct tag) yabH(SD)-F: aagaagctTGGAGAAGTAGGTGAAAGC
Reverse Primer:(cgaggcagatctctcgag tag) yabH-R: cgaggcagatctctcgagTGCTAATGCAGTTCCGCC
Length of the Cloned Region: -20 to 444 Length: 465
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YABHp