Data for the Constructed Mutant: VEGdd

General Information

Name of the MutantVEGdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHinDIII-XhoI & XhoI-BamHI
Gene Size258 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for VEGdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcg tag) veg-UF:ctcctcgAGCTTGTTATTTTTGCGGGG
Reverse Primer:(ggaggat tag) veg-UR:ggaggatCGGACAACGTCTTCGCCATT
Downstream:
Forward Primer:(aagaagc tag) veg-DF:aagaagcTTTTAACGGGCAGTGAACCT
Reverse Primer:(ctcctcg tag) veg-DR: ctcctcgAGGATGAACATATCCATA
Linearization:
Length of the Deletion: 19 to 256 Length: 238


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of VEGdd