Data for the Constructed Mutant: KSGAd

General Information

Name of the MutantKSGAd (disruption)
Used VectorpMutin1
Used Enzyme SitesBglII & EcoRI, BglII
Gene Size876 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for KSGAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) ksgA-F: cgaggcagatctTGCGACACCGATTAGAAC
Reverse Primer:(cgaggcagatctgaattc tag) ksgA-R: cgaggcagatctgaattcAATCCCTTTCAGCGGAAG
Length of the Cloned Region: 15 to 450 Length: 436
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of KSGAd