Data for the Constructed Mutant: YABFd

General Information

Name of the MutantYABFd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size558 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YABFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagc tag) yabF-F: aagaagcTTAAAGAGATCATTGTGGTC
Reverse Primer:(ggaggat tag) yabF-R: ggaggatCCTCTCGTCTTCTGGGCCAA
Length of the Cloned Region: 8 to 149 Length: 142
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YABFd