Data for the Constructed Mutant: YABFd
General Information
Name of the Mutant
YABFd (disruption)
Host
168
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
558 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YABFd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagaagc
tag)
yabF-F:
aagaagcTTAAAGAGATCATTGTGGTC
Reverse Primer:
(
ggaggat
tag)
yabF-R:
ggaggatCCTCTCGTCTTCTGGGCCAA
Length of the Cloned Region: 8 to 149 Length: 142
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:yes
Enzyme:
Probe used:
Figure
Comment: ;.
1st Level Phenotype Analysis of
YABFd