Data for the Constructed Mutant: YABAdd

General Information

Name of the MutantYABAdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-XhoI & XhoI-BamHI
Gene Size357 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YABAdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcct tag) yabA-UF:ctcctCGAGTCGACTTTAGAGAGCTTG
Reverse Primer:(ggagg tag) yabA-UR:ggaggATCCAAGGTTCCACACCTCG
Downstream:
Forward Primer:(aagaagc tag) yabA-DF:aagaagcTTGTCTTTTCTGTCTGTCATTC
Reverse Primer:(ctcctc tag) yabA-DR: ctcctcGAGATGCTCGTTCATGTTTTG
Linearization:
Length of the Deletion: 6 to 324 Length: 319


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YABAdd