Data for the Constructed Mutant: YABAdd
General Information
Name of the Mutant
YABAdd (deletion (double-cross))
Host
168
Used Vector
pMutinT3
Used Enzyme Sites
HindIII-XhoI & XhoI-BamHI
Gene Size
357 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YABAdd (deletion (double-cross))
[Construction of the Mutant]
Primers used for Deletion:
Upstream:
Forward Primer:
(
ctcct
tag)
yabA-UF:
ctcctCGAGTCGACTTTAGAGAGCTTG
Reverse Primer:
(
ggagg
tag)
yabA-UR:
ggaggATCCAAGGTTCCACACCTCG
Downstream:
Forward Primer:
(
aagaagc
tag)
yabA-DF:
aagaagcTTGTCTTTTCTGTCTGTCATTC
Reverse Primer:
(
ctcctc
tag)
yabA-DR:
ctcctcGAGATGCTCGTTCATGTTTTG
Linearization:
Length of the Deletion: 6 to 324 Length: 319
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:yes
Enzyme:
Probe used:
Figure
Comment: ;.
1st Level Phenotype Analysis of
YABAdd