Data for the Constructed Mutant: HOLBp

General Information

Name of the MutantHOLBp (promoter fusion)
Used VectorpMutin1
Used Enzyme SitesEcoRI and BamHI
Gene Size987 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for HOLBp (promoter fusion)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(gaagaatt tag) holB(SD)-F: gaagaattCTTTACACATAGAGAGTG
Reverse Primer:(ggagga tag) holB-R: ggaggaTCCGCGCCACCCTCCAGAC
Length of the Cloned Region: -23 to 182 Length: 206
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of HOLBp