Data for the Constructed Mutant: XPACd

General Information

Name of the MutantXPACd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size612 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for XPACd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagc tag) xpaC-F: aagaagcTTTTTTCACTTTTTGGTG
Reverse Primer:(taatacgactcactatagggcgagga tag) xpaC-R: taatacgactcactatagggcgaggaTCCTCTAAGTTTTTCTTA
Length of the Cloned Region: 10 to 236 Length: 227
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of XPACd