Data for the Constructed Mutant: CSFBdd

General Information

Name of the MutantCSFBdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-XhoI & XhoI-BamHI
Gene Size192 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:
Correspondence:


Mutant Construction for CSFBdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctc tag) yaaM-UF:ctcctcGAGATTTCCCATTCCGCAAG
Reverse Primer:(ggaggatc tag) yaaM-UR:ggaggatcCTGTTTCGTCCATCTTCTCC
Downstream:
Forward Primer:(aagaagc tag) yaaM-DF:aagaagcTTCATAGACCTGAAAAGGTC
Reverse Primer:(ctcctc tag) yaaM-DR: ctcctcGAGATAAATCCTTCTCACAA
Linearization:
Length of the Deletion: 13 to 189 Length: 177


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of CSFBdd