Data for the Constructed Mutant: YAALdd

General Information

Name of the MutantYAALdd (deletion (double-cross))
Host168
Used VectorpMutinT3
Used Enzyme SitesHindIII-XhoI & XhoI-BamHI
Gene Size222 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes
Correspondence:


Mutant Construction for YAALdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ctcctcg tag) yaaL-UF:ctcctcgAGGTATGAAAGAAGATGTAG
Reverse Primer:(ggaggat tag) yaaL-UR:ggaggatCCCATGGATCGCTTTTTCCT
Downstream:
Forward Primer:(gaagaat tag) yaaL-DF:gaagaatTCAGCCGGTGGAAGTAAGTG
Reverse Primer:(ctcctcg tag) yaaL-DR: ctcctcgAGCGACTTTATTATCATAAC
Linearization:
Length of the Deletion: 5 to 209 Length: 205


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YAALdd