Data for the Constructed Mutant: YAAFd

General Information

Name of the MutantYAAFd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size651 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YAAFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagct tag) yaaF-F: aagaagctTCATATCCCTAAGAATTCAA
Reverse Primer:(ggagga tag) yaaF-R: ggaggaTCCAACGTTCAAAATCATGA
Length of the Cloned Region: 12 to 175 Length: 164
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YAAFd