Data for the Constructed Mutant: YAAEd

General Information

Name of the MutantYAAEd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size588 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:yes

Mutant Construction for YAAEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagct tag) yaaE-F: aagaagctTGTACTAGGACTTCAAGG
Reverse Primer:(ggagga tag) yaaE-R: ggaggaTCCATGAATTGATACGTA
Length of the Cloned Region: 15 to 188 Length: 174
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YAAEd