Data for the Constructed Mutant: DACAd

General Information

Name of the MutantDACAd (disruption)
Used VectorpMutin1
Used Enzyme SitesBglII & EcoRI,BglII
Gene Size1329 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:

Mutant Construction for DACAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(cgaggcagatct tag) dacA-F: cgaggcagatctAAGAAATGTAAACAGCTAC
Reverse Primer:(cgaggcagatctgaattc tag) dacA-R: cgaggcagatctgaattcACTTAGTTTCAGAACCGG
Length of the Cloned Region: 10 to 448 Length: 439
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of DACAd