Data for the Constructed Mutant: YAACd
General Information
Name of the Mutant
YAACd (disruption)
Host
168
Used Vector
pMutinT3
Used Enzyme Sites
HindIII & BamHI
Gene Size
945 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
yes
Mutant Submission to NIG:
yes
Correspondence:
Mutant Construction for YAACd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
aagaagct
tag)
yaaC-F:
aagaagctTATCATGAGTGGAAAGACTT
Reverse Primer:
(
ggaggatc
tag)
yaaC-R:
ggaggatcCTAAGGGAGAATATGCGGCT
Length of the Cloned Region: 7 to 193 Length: 187
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:yes
Enzyme: EcoRV
Probe used: vector and insert
Figure
Comment: ;.
1st Level Phenotype Analysis of
YAACd