Data for the Constructed Mutant: YAACd

General Information

Name of the MutantYAACd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII & BamHI
Gene Size945 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:yes

Mutant Submission to NIG:yes

Mutant Construction for YAACd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagct tag) yaaC-F: aagaagctTATCATGAGTGGAAAGACTT
Reverse Primer:(ggaggatc tag) yaaC-R: ggaggatcCTAAGGGAGAATATGCGGCT
Length of the Cloned Region: 7 to 193 Length: 187
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YAACd