Data for the Constructed Mutant: JAGd

General Information

Name of the MutantJAGd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size624 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for JAGd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) jag-F: aagaagcttAGGAATGTGACTGCTGCAG
Reverse Primer:(ggaggatcc tag) jag-R: ggaggatccTTCTTGAACAGTCACATC
Length of the Cloned Region: 4 to 255 Length: 252
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of JAGd