Data for the Constructed Mutant: THDFd
General Information
Name of the Mutant
THDFd (disruption)
Host
Used Vector
pMutin1
Used Enzyme Sites
HindIII and BamHI
Gene Size
1377 (nt)
Growth & Expression (reporter lacZ):
in DSM:
no
in MM:
no
Mutant Submission to NIG:
no
Correspondence:
Mutant Construction for THDFd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
ctatacagccaagctt
tag)
thdF R1.1:
ctatacagccaagcttTGCGGCAATTTCCACACCGA
Reverse Primer:
(
cgcattatggggatcc
tag)
thdF F2:
cgcattatggggatccCCGGTCCGTTTTCGCTCTG
Length of the Cloned Region: 12 to 429 Length: 418
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
THDFd