Data for the Constructed Mutant: THDFd

General Information

Name of the MutantTHDFd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1377 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:no

Mutant Construction for THDFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ctatacagccaagctt tag) thdF R1.1: ctatacagccaagcttTGCGGCAATTTCCACACCGA
Reverse Primer:(cgcattatggggatcc tag) thdF F2: cgcattatggggatccCCGGTCCGTTTTCGCTCTG
Length of the Cloned Region: 12 to 429 Length: 418
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of THDFd