Data for the Constructed Mutant: GIDAd

General Information

Name of the MutantGIDAd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size1884 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:no

Mutant Construction for GIDAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ctatacagccaagctt tag) gidA R: ctatacagccaagcttAAGCAGGCCAATACGATGTG
Reverse Primer:(cgcattatggggatcc tag) gidA F: cgcattatggggatccAAGACAGATCGCCAAGAATG
Length of the Cloned Region: 11 to 511 Length: 501
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of GIDAd