Data for the Constructed Mutant: GIDBd

General Information

Name of the MutantGIDBd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size717 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:no

Mutant Construction for GIDBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ctatacagccaagctt tag) gidB R: ctatacagccaagcttATTCACTTCTGGTTTGGCGG
Reverse Primer:(cgcattatggggatcc tag) gidB F: cgcattatggggatccAGCTCTGTCATGGCAGAATG
Length of the Cloned Region: 15 to 384 Length: 370
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of GIDBd