Data for the Constructed Mutant: YYAAd

General Information

Name of the MutantYYAAd (disruption)
Used VectorpMutin1
Used Enzyme SitesHindIII and BamHI
Gene Size849 (nt)

Growth & Expression (reporter lacZ):
in DSM:no
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYAAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ctatacagccaagctt tag) yyaA R: ctatacagccaagcttCATTCTCTCGTTTCTTCGGG
Reverse Primer:(cgcattatgcggatcc tag) yyaA F: cgcattatgcggatccGAGTTCGTATTGGCCTTCTT
Length of the Cloned Region: 11 to 249 Length: 239
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYAAd