Data for the Constructed Mutant: YYABd

General Information

Name of the MutantYYABd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size438 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYABd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaB-F: aagaagcttGTTTATCAAACGAAGAGAG
Reverse Primer:(ggaggatcc tag) yyaB-R: ggaggatccGATCAGATGGTGTTCCTTC
Length of the Cloned Region: 4 to 210 Length: 207
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYABd