Data for the Constructed Mutant: YYACd

General Information

Name of the MutantYYACd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size615 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYACd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaC-F: aagaagcttCGAAAAGGCGGGCTTTTTTCTTC
Reverse Primer:(ggaggatcc tag) yyaC-R: ggaggatccGGGGTCAGATAAGGTTCC
Length of the Cloned Region: 7 to 255 Length: 249
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYACd