Data for the Constructed Mutant: YYADd

General Information

Name of the MutantYYADd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1014 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYADd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaD-F: aagaagcttATGCTGCTCATATTAGGG
Reverse Primer:(ggaggatcc tag) yyaD-R: ggaggatccTTTCGCCAGCCATGGCCC
Length of the Cloned Region: 28 to 240 Length: 213
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYADd