Data for the Constructed Mutant: YYAEd

General Information

Name of the MutantYYAEd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size2001 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YYAEd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaE-F: aagaagcttAGCAAAGTGCATCAGTCG
Reverse Primer:(ggaggatcc tag) yyaE-R: ggaggatccAATTTCATCATCAAGTGCCTG
Length of the Cloned Region: 4 to 2005 Length: 2002
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YYAEd