Data for the Constructed Mutant: EXOAd

General Information

Name of the MutantEXOAd (disruption)
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size756 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for EXOAd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) exoA-F: aagaagcttAAGTTGATTTCATGGAATG
Reverse Primer:(ggaggatcc tag) exoA-R: ggaggatccCTCTTCTACTCCGATACC
Length of the Cloned Region: 4 to 255 Length: 252
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of EXOAd