Data for the Constructed Mutant: YYAHd

General Information

Name of the MutantYYAHd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size378 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYAHd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaH-F: aagaagcttCAGATTGAACATACAGCG
Reverse Primer:(ggaggatcc tag) yyaH-R: ggaggatccTTTCTCATTATGATACAAATC
Length of the Cloned Region: 4 to 108 Length: 105
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYAHd