Data for the Constructed Mutant: YYALd

General Information

Name of the MutantYYALd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size2067 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYALd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(ggaggatcc tag) yyaL-F: ggaggatccAGTAAACCCAATAGATTAATC
Reverse Primer:(ggaggatcc tag) yyaL-R: ggaggatccGATAAACACATTCAGCGG
Length of the Cloned Region: 13 to 345 Length: 333
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYALd