Data for the Constructed Mutant: YYAMd

General Information

Name of the MutantYYAMd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size915 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYAMd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaM-F: aagaagcttATAATGTTACTTGGCTTCTC
Reverse Primer:(ggaggatcc tag) yyaM-R: ggaggatccTGCTCCGTTAACGGGAGATG
Length of the Cloned Region: 22 to 294 Length: 273
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYAMd