Data for the Constructed Mutant: YYAOdd

General Information

Name of the MutantYYAOdd (deletion (double-cross))
Host
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size237 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no
Correspondence:


Mutant Construction for YYAOdd (deletion (double-cross))

[Construction of the Mutant]

Primers used for Deletion:
Upstream:
Forward Primer:(ggaggatcc tag) yyaO-P1:ggaggatccCATGGGATCACCTTCTTCC
Reverse Primer:(ctcctcgag tag) yyaO-P2:ctcctcgagAAATCCAATGATCGACCC
Downstream:
Forward Primer:(aagaagctt tag) yyaO-P3:aagaagcttTTCAAACAATACAGTCTAC
Reverse Primer:(ctcctcgag tag) yyaO-P4: ctcctcgagCAAAATTGAATAATATGTATCCAG
Linearization:
Length of the Deletion: 403 to -238 Length: 642


[Confirmation of the Constructed Mutant]


1st Level Phenotype Analysis of YYAOdd