Data for the Constructed Mutant: YYAOdd
General Information
Name of the Mutant
YYAOdd (deletion (double-cross))
Host
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
237 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
no
Correspondence:
Mutant Construction for YYAOdd (deletion (double-cross))
[Construction of the Mutant]
Primers used for Deletion:
Upstream:
Forward Primer:
(
ggaggatcc
tag)
yyaO-P1:
ggaggatccCATGGGATCACCTTCTTCC
Reverse Primer:
(
ctcctcgag
tag)
yyaO-P2:
ctcctcgagAAATCCAATGATCGACCC
Downstream:
Forward Primer:
(
aagaagctt
tag)
yyaO-P3:
aagaagcttTTCAAACAATACAGTCTAC
Reverse Primer:
(
ctcctcgag
tag)
yyaO-P4:
ctcctcgagCAAAATTGAATAATATGTATCCAG
Linearization:
Length of the Deletion: 403 to -238 Length: 642
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YYAOdd