Data for the Constructed Mutant: YYAQd

General Information

Name of the MutantYYAQd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size354 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYAQd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaQ-F: aagaagcttTTAACAAGAGAAGATATC
Reverse Primer:(ggaggatcc tag) yyaQ-R: ggaggatccATTCATGATAAGACCGTACC
Length of the Cloned Region: 4 to 138 Length: 135
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYAQd