Data for the Constructed Mutant: YYARd

General Information

Name of the MutantYYARd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size519 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYARd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaR-F: aagaagcttACTCATTTGAATATGAAAG
Reverse Primer:(ggaggatcc tag) yyaR-R: ggaggatccAGCCGCTTTCCCCTCTTC
Length of the Cloned Region: 16 to 201 Length: 186
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYARd