Data for the Constructed Mutant: YYASd

General Information

Name of the MutantYYASd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size603 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYASd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yyaS-F: aagaagcttTACGGAATAGGCATACTC
Reverse Primer:(ggaggatcc tag) yyaS-R: ggaggatccTGCTGTAATCAAGCCCAG
Length of the Cloned Region: 16 to 228 Length: 213
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYASd