Data for the Constructed Mutant: YYATd
General Information
Name of the Mutant
YYATd (disruption)
Host
Used Vector
pMutinT3
Used Enzyme Sites
HindIII and BamHI
Gene Size
444 (nt)
Growth & Expression (reporter lacZ):
in DSM:
yes
in MM:
no
Mutant Submission to NIG:
no
Correspondence:
Mutant Construction for YYATd (disruption)
[Construction of the Mutant]
Primers used for Cloning:
Forward Primer:
(
tag)
yyaT-F:
CATGAAGCTTTAAAGATTAG
Reverse Primer:
(
ggaggatcc
tag)
yyaT-R:
ggaggatccCCCCAATCCGTACTTACG
Length of the Cloned Region: -444 to -444 Length: 1
+1 is the first nucleotide of the putative initiation codon
[Confirmation of the Constructed Mutant]
Confirmation of the Constructed Mutant by PCR:
Confirmation of the Constructed Mutant by Southern:
1st Level Phenotype Analysis of
YYATd