Data for the Constructed Mutant: YYBBd

General Information

Name of the MutantYYBBd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size660 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBBd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybB-F: aagaagcttCAATCAGAACATGTC
Reverse Primer:(ggaggatcc tag) yybB-R: ggaggatccACCCCCGATATGATCACTG
Length of the Cloned Region: 664 to 225 Length: 440
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBBd