Data for the Constructed Mutant: YYBCd

General Information

Name of the MutantYYBCd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size477 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBCd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybC-F: aagaagcttGCAAATAAAAAAGAAGGC
Reverse Primer:(ggaggatcc tag) yybC-R: ggaggatccGATCATAAAACCGATGAC
Length of the Cloned Region: 7 to 153 Length: 147
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBCd