Data for the Constructed Mutant: YYBDd

General Information

Name of the MutantYYBDd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size441 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBDd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybD-F: aagaagcttAAAATCACGTCGGAACAGG
Reverse Primer:(ggaggatcc tag) yybD-R: ggaggatccACGGACACGTGCAGTGCC
Length of the Cloned Region: -441 to -441 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBDd