Data for the Constructed Mutant: YYBFd

General Information

Name of the MutantYYBFd (disruption)
Used VectorpMutinT3
Used Enzyme SitesHindIII and BamHI
Gene Size1212 (nt)

Growth & Expression (reporter lacZ):
in DSM:yes
in MM:no

Mutant Submission to NIG:no

Mutant Construction for YYBFd (disruption)

[Construction of the Mutant]

Primers used for Cloning:
Forward Primer:(aagaagctt tag) yybF-F: aagaagcttGGAATTGTTGGTTTGCCG
Reverse Primer:(ggaggatcc tag) yybF-R: ggaggatccGTCATCGGAAAAAGCACG
Length of the Cloned Region: 1216 to 1216 Length: 1
+1 is the first nucleotide of the putative initiation codon

[Confirmation of the Constructed Mutant]

1st Level Phenotype Analysis of YYBFd